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GenCh9answers - Proteins and Their Translation BASIC...

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Proteins and Their Translation B ASIC P ROBLEMS 1. 3 ´ CGT ACC ACT GCA 5 ´ DNA double helix (transcribed strand) 5 ´ GCA TGG TGA CGT 3 ´ DNA double helix 5 ´ GCA UGG UGA CGU 3 ´ mRNA transcribed 3 ´ CGU ACC ACU GCA 5 ´ appropriate tRNA anticodon NH 3 - Ala - Trp - (stop) - COOH amino acids incorporated 2. a. and b. 5 ´ UUG GGA AGC 3 ´ c. and d. Assuming the reading frame starts at the first base: NH 3 - Leu - Gly - Ser - COOH For the bottom strand, the mRNA is 5 ´ GCU UCC CAA 3 ´ and assuming the reading frame starts at the first base, the corresponding amino acid chain is NH 3 - Ala - Ser - Gln - COOH. 3. (5) With an insertion, the reading frame is disrupted. This will result in a drastically altered protein from the insertion to the end of the protein (which may be much shorter or longer than wild type because of altered stop signals). 6. There are three codons for isoleucine: 5´ AUU 3´, 5´ AUC 3´, and 5´ AUA 3´. Possible anticodons are UAA (complementary), UAG (complementary), and 3´ UAI 5´ (wobble). 5´ UAU 3´, although complementary,
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