Lecture - Listeria monocytogenes

Lecture - Listeria monocytogenes - LISTERIA MONOCYTOGENES...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: LISTERIA MONOCYTOGENES Listeriosis has emerged as one of the major foodborne diseases during the last decade. Listeriosis is an atypical foodborne disease of major public health concern because of the severity and non-enteric nature of the disease (meningitis, septicemia and abortion), a high case fatality rate, long incubation time, and risk groups when infected leads to T-cell-mediated immunity. P la c e 2 5 g o f s a m p le in to 2 2 5 m l o f U V M b r o th . I n c u b a te a t 3 0 C fo r 2 4 h o u r s T r a n s fe r 0 .1 m l o f in o c u la te d U V M b r o th to 1 0 m l o f F r a s e r B r o th @ 3 5 fo r 2 4 h o u r s T r a n s fe r d a r k e n e d tu b e s o n to M O X a g a r p la te s . I n c u b a te @ 3 5 C fo r 2 4 h o u r s L i s t e r i a s p p . y ie ld s b la c k z o n e s a r o u n d e a c h c o lo n y w h e n g r o w n o n M O X T r a n s fe r ty p ic a l c o lo n ie s o n to H o r s e B lo o d a g a r a n d in c u b a te fo r 2 4 h o u r s @ 3 5 C . H o r s e B lo o d a g a r d iffe r e n tia te s L . m o n o c y t o g e n e s fr o m o th e r ty p e s o f L i s t e r i a s p p . L . m o n o c y t o g e n e s c o lo n ie s s h o w - h e m o ly s is , a c le a r in g z o n e a r o u n d e a c h c o lo n y . T r a n s fe r o n e c o lo n y in to m o tility a g a r . L i s t e r i a s p p . s h o w a d is tin c tiv e u m b r e lla m o tility . M o tility a g a r c o n fir m s th e s p e c ie s a s L i s t e r i a Listeria: Characteristics of the organism Within the genus of Listeria, only L....
View Full Document

Page1 / 19

Lecture - Listeria monocytogenes - LISTERIA MONOCYTOGENES...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online