PCB3134 - Lecture 2 Techniques in Cell and Molecular Biology

PCB3134 - Lecture 2 Techniques in Cell and Molecular...

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Bacteria (Eubacteria) Archaea (Archeabacteria) Eukarya Informational processes Metabolism B B B B
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Techniques in Cell and Molecular Biology Microscopy The light microscopy Phase-contrast microscopy Fluorescence microscopy Confocal scanning light microscopy Transmission and scanning electron microscopy Freeze-refraction replication (Chapter 18, p733-780) Pulse-chase experiment Differential centrifugation Polyacrylamide gel electrophoresis X-ray crystallography Immunolocalization
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The Light Microscopy
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The Light Microscopy
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Empty Magnification
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Resolution d = 0.61 x n sin x Numerical Aperture ( N . A .): n sin n is the refractive index of the medium. The optical quality of an objective lens is measured by its resolution.
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Diffraction The resolution obtained by a microscope is limited by diffraction.
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Visibility and Contrast Refractive index: n A part of a specimen can be seen because it affects the light differently from its adjacent parts and its background Oil
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Phase-Contrast Microscope (interference microscope)
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PCB3134 - Lecture 2 Techniques in Cell and Molecular...

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