PCB3134 - Lecture 22 Smooth ER and Rough ER

PCB3134 - Lecture 22 Smooth ER and Rough ER - C HAPTER 8 C...

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Under light microscope, plasma membrane, mitochondrion, chloroplast, and nucleus can be easily distinguished, whereas the cytoplasm of living cells appears relatively devoid of structure. CHAPTER 8 Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking Under electron microscope, the cytoplasm was found full of diverse array of membrane-bound structures, including endoplasmic reticulum (ER), Golgi complex, endosomes, lysosomes, and vacuoles. These organelles form an endomembrane system in which the individual components function as part of a coordinated unit. The endomembrane system does not include mitochondria, peroxisomes, and chloroplasts.
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A maize root cell, showing the membrane-bound compartments of the cytoplasm
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The organelles of the endomembrane system are part of a dynamic integrated network in which materials are shuttled back and forth from one part of the cell to another. Materials are shuttle in small membrane-bounded transport vesicles.
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Dynamic property of the endomembrane system Biosynthetic/secretory pathway Constitutive secretion Regulated secretion Endocytic pathway
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Approaches to the study of endomembranes Autoradiography: “pulse-chase” experiment Use of the green fluorescent protein Biochemical analysis of subcellular fractions Use of cell-free systems Study of genetic mutants
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The Use of Radioisotope - Pulse-Chase Experiment Definition: An experiment in which cells are grown in radioactive medium for a brief period (the pulse) and then transferred to nonradioactive medium for a longer period (the chase). Radioactive chemicals (Pulse) Unlabeled chemicals (Chase) Analysis
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The movement of labeled secretory proteins (red) after 3-minute pulse and 17-minute or 117-minute chase
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After 3-minute pulse, the labeled proteins are localized over the ER.
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Endoplasmic reticulum is an extensive network of membranes that extends from the cell membrane through the cytoplasm to the nuclear membrane. There are two types of ER, smooth ER
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PCB3134 - Lecture 22 Smooth ER and Rough ER - C HAPTER 8 C...

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