Bi1_2009_Lecture23_full

Or introduce random mutations into variable regions

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Unformatted text preview: spleen DNA. Or introduce random mutations into variable regions genes of a specific antibody. Clone into a phage so Multiply phage that each phage display library in expresses one bacteria, bind phage VH-VL surface fusion to surface coated protein. with antigen. Wash away unbound phage. Repeat procedure (multiply recovered phage, bind to antigen, wash away unbound phage) for several cycles. Recover specific high-affinity antigen binding VH-VL regions. Phage Display schematic 1 3 2 4 5 6 7 8 Phage display to select for sequence-specific DNA binding proteins • Zinc finger proteins are modular. • Each finger contains two anti-parallel -strands, an -helix, and a...
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This note was uploaded on 09/25/2010 for the course BIO 1 taught by Professor Bakorman during the Spring '09 term at Caltech.

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