Or introduce random mutations into variable regions

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: spleen DNA. Or introduce random mutations into variable regions genes of a specific antibody. Clone into a phage so Multiply phage that each phage display library in expresses one bacteria, bind phage VH-VL surface fusion to surface coated protein. with antigen. Wash away unbound phage. Repeat procedure (multiply recovered phage, bind to antigen, wash away unbound phage) for several cycles. Recover specific high-affinity antigen binding VH-VL regions. Phage Display schematic 1 3 2 4 5 6 7 8 Phage display to select for sequence-specific DNA binding proteins • Zinc finger proteins are modular. • Each finger contains two anti-parallel -strands, an -helix, and a...
View Full Document

This note was uploaded on 09/25/2010 for the course BIO 1 taught by Professor Bakorman during the Spring '09 term at Caltech.

Ask a homework question - tutors are online