Bi1_2009_Lecture23_full

The helix from each finger inserts into the major

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Unformatted text preview: Zn atom. • The -helix from each finger inserts into the major groove of DNA. A General Strategy for Selecting High Affinity Zinc Finger Proteins for Diverse DNA Target Sites Greisman et al., 1997, Science 275: 657 First use 3finger/DNA crystal structure to determine important protein/DNA contacts A General Strategy for Selecting High Affinity Zinc Finger Proteins for Diverse DNA Target Sites Greisman et al., 1997, Science 275: 657 Originally bound to this sequence RNA aptamers -- antibody-like properties Aptamers have been made against small molecules, peptides, proteins, organelles, viruses, cells Vitamin b12binding RNA aptamer Aptamer database: http://aptam...
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This note was uploaded on 09/25/2010 for the course BIO 1 taught by Professor Bakorman during the Spring '09 term at Caltech.

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