Unformatted text preview: er.icmb.utexas.edu/index.php Let the immune system make enzymes for you Catalyst must bind more tightly to transition state than to products or reactants. Catalytic Antibodies
• Raise antibodies against a transition state analog • Screen hybridomas for antibodies that catalyze desired reaction Wedemayer et al. (1997) Science 276, 1665-1669. Catalytic antibody that hydrolyzes cocaine
Zhu et al., 2006, Structure 14: 205-216 (Nonpsychoactive products) Compound that was injected to raise antibodies Transition state analog that was crystallized with Fab / barrel enzymes evolved from a common ancestor 10% of enzymes are / barrels But this takes a long time… Directed evolution http://ocw.m...
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