1aa3-2010-tut12 - CHEMISTRY 1AA3 April 5-8, 2010 TUTORIAL...

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CHEMISTRY 1AA3 April 5-8, 2010 TUTORIAL PROBLEM SET 12 – QUESTIONS ______________________________________________________________________________ 1 Questions 1 - 4: Some practical enzyme kinetics (from the lecture) Our diet contains many complex polysaccharides (polymers of different sugars), which must be broken down to monosaccharides in order to be converted to energy. Polysaccharides are broken down through a variety of routes, which includes significant amounts of disaccharide formation. Let's consider three disaccharides, cellobiose, gentiobiose and lactose, that must be hydrolyzed to the corresponding monosaccharides, glucose & galactose. A hypothetical enzyme, glycosidase X (gX), which catalyzes this reaction. gX will be digest each disaccharide with different steady state rate constants. 1. (a) With gentiobiose: k cat = 10 s -1 and K M = 10 -4 M. If: [gX] = 10 -7 M, and[gentiobiose] = 10 -5 M, what is v 0 ? (b) If [gentiobiose] increases to 2 × 10 -5 M, what is the new v 0 ? (c) Why does the rate roughly double when [gentiobiose] doubles? 2. (a) If v 0 = 2 × 10 -6 M/s with 4 × 10 -4 M cellobiose and 1.8 × 10 -6 M/s with 2 × 10 -4 M cellobiose, what can we conclude about K M (cellobiose)? (b)
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1aa3-2010-tut12 - CHEMISTRY 1AA3 April 5-8, 2010 TUTORIAL...

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