10 LECTURE 6 and 7 BIO 325

10 LECTURE 6 and 7 BIO 325 - Why do cells on the outside of...

Info iconThis preview shows pages 1–10. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Why do cells on the outside of the compacted morula differentiate into trophoblast cells? Model of cell position-dependent fate speciFcation in pre-implantation embryos In the polarized outer cells, hippo signaling is supressed. Yap increases and together with Tead4 activates expression of trophoblast speciFc genes ( Cdx2) Increased cell contact activates hippo signaling – triggering phosphorylation and degradation of Yap. Tead4 without Yap can not Model of cell position-dependent fate speciFcation in pre-implantation embryos In the polarized outer cells, hippo signaling is supressed. Yap increases and together with Tead4 activates expression of trophoblast speciFc genes ( Cdx2) Increased cell contact activates hippo signaling – triggering phosphorylation and degradation of Yap. Tead4 without Yap can not activate trophoblast genes Cdx2 + Oct4 TEAD4 + Oct4 TEAD4 +/+ TEAD4 -/- TEAD4 mutants do not express Cdx2 (a homeodomain transcription factor) that regulates genes needed for trophoblast differentiation. http://www.rikenresearch.riken.jp/eng/frontline/5854 Overexpression of Tead4 activates Cdx2 in ES cells and triggers trophoblast differentiation in cell culture What affect does overexpression of Tead4 have in the embryo? Approach: inject mRNA coding for an activated Tead4 (Tead4VP16) and test to see if Cdx2 becomes activated in inside cells. 16% 78% 6% 37% Nuclear Yap overlaps with Cdx2 expression Nuclear Yap anticipates expression of Cdx2 a trophoblast gene There are two Lats genes. If both genes are simultaneously knocked out, Yap is located in the nucleus of all cells. Cdx2 becomes activated in all cells How will this affect cell differentiation?? If Lats2 is overexpressed, Yap does not accumulate in the nucleus and Cdx2 is not activated. Cell contact and cell polarity infuence Yap localization Dissagregation oF 8-cell blastomeres triggers a change in Yap localization Reaggregation oF two 8-cell embryos – Nuclear Yap in polarized cells. Model of cell position-dependent fate speciFcation in...
View Full Document

{[ snackBarMessage ]}

Page1 / 37

10 LECTURE 6 and 7 BIO 325 - Why do cells on the outside of...

This preview shows document pages 1 - 10. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online