FLOPED-MATER-TLE6 2008 Dev Cell supp

FLOPED-MATER-TLE6 - Developmental Cell 15 Supplemental Data A Subcortical Maternal Complex Essential for Preimplantation Mouse Embryogenesis Lei Li

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Developmental Cell 15 Supplemental Data A Subcortical Maternal Complex Essential for Preimplantation Mouse Embryogenesis Lei Li, Boris Baibakov, and Jurrien Dean Supplemental Experimental Procedures Probes and Primers P1: 5’-CCCTGCTGACAGTGGACTC-3’; P2: 5’-CCAGCCAGTTTTAGCCCTTT-3’; P3: 5’- TGCGCAACTGTTGGGAAG-3’) were used for genotyping. TaqMan ® probes and gene-specific primers (Mm00509585_gl, Mm00475107_m1, Mm00488691_ml; Applied Biosystems, Foster City, CA USA) were used for qRT-PCR. The DIG-labeled oligonucleotide antisense sequences used for in situ hybridization were 5'- GGAACCACCAGGGCCGAGTGCGAAGTCTCAGGGACACAGGCAGGACGT-3' and 5'- CCCGGGGTGCTTTTGAACTTGGGGTGCTGGAGGGCTGCAGACTCTGGG-3' for Floped and Tle6 , respectively. Antibodies Antibodies used for immunofluorescence and immunoblots included: rabbit anti-FLOPED (1 μg/ml), rabbit anti-TLE6 (1 μg/ml), sheep anti-Filia (1 μg/ml), rabbit anti-MATER (0.2 μg/ml), mouse anti-OCT4 (Santa Cruz Biotechnology, 1:100), A5441- monoclonal antibody to
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This note was uploaded on 09/29/2010 for the course BIO 325 taught by Professor Bernadette-holdener,g during the Fall '08 term at SUNY Stony Brook.

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FLOPED-MATER-TLE6 - Developmental Cell 15 Supplemental Data A Subcortical Maternal Complex Essential for Preimplantation Mouse Embryogenesis Lei Li

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