Lect18 - Lecture 18 Protein Degradation 1 Protein Food...

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1 Lecture 18 Protein Degradation
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2 Protein Food Protein intracellular Amino acids Metabolic Fuel (Acetyl-CoA, Pyruvate, etc) Proteins
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3 Digestion of dietary proteins
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Amylase, the enzyme that degrades starch in Saliva has a pH optima of 7.0. Pepsin is a digestive protease works has a pH optima of pH 2-3 . What residue do you think is involved in catalysis? Where do you think it is active? Trypsin has a pH optima of 8.0, Where do you think it is active? Because enzymes and other proteins have an optimum pH range they become inactivated or denatured outside this range
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5 Protein Food Protein intracellular Amino acids Metabolic Fuel (Acetyl-CoA, Pyruvate, etc) Proteins
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6 Regulated Degradation: Ubiquitin-Proteasome System Aaron Ciechanover Avram Hershko Irwin Rose Nobel Prize in Chemistry, 2004 Alex Varshavsky Only 3 people can win the Nobel Prize …
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Ubiquitenation functions • Regulation of cellular signals. • Removal of misfolded proteins. • Removal of damaged proteins.
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8 Poly-ubiquitin (Usually Lys-48 one ubiquitin to C-terminus of another) •76 residues, highly conserved •C terminus attached to Lys in the protein to be degraded What is Ubiquitin
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9
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10 Regulated Degradation: Ubiquitin-Proteasome System Sulfhydryl group of a Cys in E1 ATP driven E1 — Ubiquitin Activating Enzyme E2 — Ubiquitin Conjugating Enzyme E3 — Ubiquitin Protein Ligase (HECT, RING)
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E3 a gate keeper of health? • Humans have hundreds of E3 members
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This note was uploaded on 09/29/2010 for the course ECON 100A taught by Professor Smith during the Spring '08 term at Alabama.

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Lect18 - Lecture 18 Protein Degradation 1 Protein Food...

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