Unformatted text preview: 4. Mix 10 microliters of each standard into 3 separate wells with the G-250 dye. This should use 15 wells 5. Mix dye and protein solution 6. Allow at least 5 minutes for incubation 7. Make sure the colors correspond to the concentration 8. Read all wells with the microplate reader set at 595 nm 9. Cover wells with tape and store 10. Record all data in lab notebook...
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- Spring '10
- pH, Microtiter plate, protein solution, Andrew Klingsporn, Biology Protein Determination