Final 2006 - Final Exam. BIO201B. 2006 form 1 Each question...

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Final Exam. BIO201B. 2006 form 1 Each question has only one right answer. You can write on this exam form if you wish. 1. If a nascent protein does not have a signal sequence then, A. It will not be translated B. It cannot become a soluble, cytoplasmic protein like tubulin C. It will be translated on free polysomes in the cytoplasm D. It will become a membrane protein somewhere in the endomembrane system E. It will bind to the translocon instead of the SRP receptor 2. Lysosomes are: A. Acidified by a lysosomal membrane proton pump B. Filled with many enzymes that were made in the cytoplasm C. Mitochodrial inner membranes that are inside-out D. Vesicles that do not contain any soluble glycoproteins E. Not part of the endomembrane system 3. Cotranslational translocation means: A. The ribosome moves along the mRNA as it is being translated on free polysomes B. Transcription and translation of a gene happening at the same time C. Movement of the tRNA from the first site to the second site on the ribosome during translation D. Movement of a nascent protein protein across the RER membrane while it is being translated E. Folding of a completed protein into its proper conformation inside the RER 4. Which of the following is not necessary for synthesis of an integral membrane protein? A. SRP B. Translocon C. Stop transfer sequence D. SRP receptor E. Transmembrane cargo receptor 5. Transition vesicles can be trafficked and targeted to their proper final destinations by all of the following except : A. COPI B. COPII C. Chaperones like BIP D. Clathrin E. v-SNARES and t-SNARES 6. In the Meselson-Stahl experiment with bacterial DNA, newly synthesized DNA was separated from parental DNA by: A. Density centrifugation B. Differential centrifugation C. Autoradiography D. Gel electrophoresis E. Changing the growth media from 15 N to 14 N 1
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7. Why was it necessary to use two rounds of replication in the Meselson-Stahl experiments with bacterial DNA? A. Because 15 N is not incorporated into DNA until the second round of replication in bacteria B. Because the bacterial DNA was circular, not linear C. Because the procedure used was not able to differentiate between dispersive and semi-conservative replication in the first round alone D. Because just using one round of replication did not produce enough DNA to see in their assay. Two rounds were necessary to get enough DNA E. Because it took two rounds to make all of the DNA have 15 N in it 8. If you added BrdU to growing bacteria, which of the following is most likely? A. It would kill the cells because BrdU only works with eukaryotic cells B. All of the newly synthesized DNA would have BrdU in it instead of thymidine (T) C. Some double-stranded DNA would be made up of all BrdU (no T) in the second generation D. One chromatid would stain dark and one would stain light after the first generation E. All of the bacterial RNA would have BrdU in it 9. The role of the transmembrane cargo receptor is: A. To change the conformation of the cargo protein so that it can be exported to
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Final 2006 - Final Exam. BIO201B. 2006 form 1 Each question...

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