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Name: Amir Abutalib Date Performed: 03/25/2020 Name of Lab Partner: Johnny Tran Date Submitted: 04/01/2020 Exp 7: Spectrophotometric Determination of Iron in Dietary Tablets
Introduction and Theoretical Background A spectrophotometer device measures the full spectrum of wavelengths and then uses slices of that spectrum to generate a model in determining the intensity of light. It is slightly different compared to a photometer. A photometer instead targets a select wavelength region to measure and compares the response with non-absorbing wavelengths. In this experiment, the light intensity of a colored iron complex will be measured and correlated with the amount of Fe standard added to the solution; the source of the iron will be from a vitamin supplement tablet. Equations for this experiment: Beer’s Law: A = abcAbsorbance = (Absorbance Std)/(Unknown) - Absorbance Blank Reactions for this experiment:
Materials and MethodologyThe following procedure outlines, step by step, how this experiment was carried out: One tablet of the iron-containing vitamin was placed in a 100-mL beaker, mixed with 25mL of 6M HCl, and boiled gently in a fume hood for about 15 minutes. Then, the solution was filtered directly into a 100mL volumetric flask. The beaker was filtered several times with small portions of water to allow a complete quantitative transfer. After the solution cooled, the flask was filled to the mark with DI water and mixed well by inverting. 5mL of the solution was then transferred and filled to the 100mL mark in a volumetric flask. 10mL of standard Fe