Microscopy discussion (use this one)

Microscopy discussion (use this one) - CBNS101 Discussion...

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CBNS 101 Discussion Section Office: LSP 2630 Office hours: Tues 3-5 pm Phone number: 951-827-3687 E-mail: [email protected]
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Purposes of the Discussion Section Reinforcement of lecture material Overview of some basic cell biology techniques E.g. microscopy, protein visualization Discussion of problem sets Problem set : a series of questions that will (hopefully) help prepare you for the exams New this quarter: selection and discussion of paper topic.
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Grading of the discussion section Attendance: - Attendance in discussion counts for 4% of your final grade. - You must attend the section you signed up for. If there is a conflict, contact both the TA of the section you normally attend and the TA of the section you would like to attend, and you may be able to attend an alternative section. Quizzes: -Quizzes will be administered at random. -Quiz questions will be related (but not identical to) material on the problem sets, and the exact same quiz will not be given to all students. -Scores will contribute 6% of your final grade, with the lowest quiz score dropped. (No make-up quizzes will be given.)
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Microscopy: The ability to see cells and their internal structures is a fundamental requirement of studying cells. There are two main types of microscopy: Light microscopy : Incident light is used to illuminate the specimen. This ranges over a wavelength spectrum of 400 to 700 nm. Electron microscopy : A beam of electrons is used to irradiate the specimen. The wavelength in this case is .004 nm.
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Resolution. How close can two objects be and still distinguished as separate? As light passes around an object the waves are diffracted. this may not affect visualization of large objects small objects may appear less distinct at some point, it will be impossible to distinguish two objects (large or small) as separate
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The resolving power of the light microscope is ~200nm. This is sufficient to visualize virtually all cells and many internal features of eucaryotic cells provided they can be sufficient stained. The resolving power of the electron microscope is ~0.1 nm. Provided sufficient contrast can be generated, this is sufficient to visualize molecular constituents of cells. The wavelength of the irradiation source is a fundament limit for resolution (the optics of the objectives come into play too).
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Even if your microscope can resolve an object, it doesn’t mean that you can see it.
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