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BIS 104 survey ans spr 2010

BIS 104 survey ans spr 2010 - Transfection transgenic...

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BIS 104 Midterm I, Monday, April 19, 2010: Potential Topics Basic cell structure and function Functions common to all plant & animal cells Differences / similarities between prokaryotes and eukaryotes Major cell organelles and components location within the cell; molecular composition; main functions Conceptual approaches to investrigating cell function Scientific method Observation Hypothesis testing Experimental design Correlation Loss-of-function Gain-of-function Controls Positive controls Negative controls Technical approaches to investigating cell function
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Unformatted text preview: Transfection; transgenic animal models Antibody-based experiments Immunoprecipitation; immuno(histo; cyto)chemistry; blocking antibodies Microscopy Light miocroscopy Bright-field; phase-contrast; fluorescence Electron microscopy Transmission EM (TEM) Biomembranes Basic structure and function Practice Questions: #1 – 12 Mukouyama: intro; Fig. 1; Fig. 2 Rules of Evidence I & II Assigned reading in Karp: 1-13; 15-17; 20-21; 120 – 130; 731-734; 769-772; 774-776...
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