BIS 104 assign week 6 spr 2010

BIS 104 assign week 6 spr 2010 - P75: a marker found on the...

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Assignment #6: week #6: May 3, 5, 7 You will need to understand the following techniques: RT-PCR; gel electrophoresis of nucleic acids; FACS (the FACS technique is not covered in Karp, so I will provide an explanation in the suggested reading folder) You will also to know about the following new molecules: GFAP (glial fibrillary acidic protein): specific to Schwann cells SCG10: specific to neurons TuJ1: specific to neurons (like 2H3) IB4-lectin: a carbohydrate marker found on the PM of neurons
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Unformatted text preview: P75: a marker found on the PM of Schwann cells (both IB4-lectin and P75 are used to fluorescently label the two cell types in the FACS procedure) Hprt: hypoxanthinephosphoribosyl transferase: an enzyme found in all cell types (used as an internal control in the gel experiments) VEGF: vascular endothelial growth factor (an angiogenic protein that acts specifically on endothelial cells; we will ignore that fact that it exists in at least 4 isoforms)....
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BIS 104 assign week 6 spr 2010 - P75: a marker found on the...

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