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LAb15 - Harjit Kaur Che 201 Ex 15 I A TLC of Analgesic...

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Harjit Kaur Che 201 Ex 15 I A . TLC of Analgesic Compounds – The purpose of this experiment was to learn about Thin Layer Chromatography. TLC is a technique used to separate mixtures. It is usually performed on a glass sheet, aluminum foil or silica gel. It is used to find the components of a mixture and it can also be used to detect pesticides in food. After the sample has been applied on the plate, a solvent or solvent mixture is drawn up the plate via capillary action . Because different compounds ascend the TLC plate at different rates, separation is achieved. II A. For this experiment we did Part B, a developing solution of ethyl acetate: acetic acid 200:1 is used. A filter paper is then added the solution to help ascend the mixture throughout the beaker. We then got 3 samples, Aspirin, Acetaminophen, Caffeine and unknown sample A. A line was drawn on the 1 cm mark and the different solutions were then drawn up and added to the TLC plate. The spotted TLC plate is then added to the developing solution.
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