In Photosynthesis Lab 4A, our group investigated whether light and active chloroplasts are required in the production of sugar in photosynthesis. To determine the effect of light on chloroplasts, we used the electron acceptor DPIP instead of the electron acceptor NADP and measured transmittance because DPIP changes from blue to a clear color as it’s reduced. As transmittance increases, photosynthesis is expected to increase since NADPH (DPIP) is one of the required reactants of dark reaction of photosynthesis or the Calvin Cycle. The independent variable in this experiment is boiled or un-boiled chloroplasts and dark or light environmental conditions, while the dependent variable is transmittance and thus the rate of photosynthesis. Before the actual experiment, our group predicted that a solution with light and un-boiled chloroplasts will have the highest transmittance.To perform this experiment, our group first created a set-up of four test tubes set up in front of a lamp that serves as a light source and beaker of water in between acting as a heat sink, eliminating the variable of heat.
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