Biology Photosynthesis Lab

Biology Photosynthesis Lab - In Photosynthesis Lab 4A, our...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: In Photosynthesis Lab 4A, our group investigated whether light and active chloroplasts are required in the production of sugar in photosynthesis. To determine the effect of light on chloroplasts, we used the electron acceptor DPIP instead of the electron acceptor NADP and measured transmittance because DPIP changes from blue to a clear color as its reduced. As transmittance increases, photosynthesis is expected to increase since NADPH (DPIP) is one of the required reactants of dark reaction of photosynthesis or the Calvin Cycle. The independent variable in this experiment is boiled or un- boiled chloroplasts and dark or light environmental conditions, while the dependent variable is transmittance and thus the rate of photosynthesis. Before the actual experiment, our group predicted that a solution with light and un-boiled chloroplasts will have the highest transmittance. To perform this experiment, our group first created a set-up of four test tubes set up in front of a lamp that serves as a light source and beaker of water in between acting as a heat sink, eliminating the variable of heat. serves as a light source and beaker of water in between acting as a heat sink, eliminating the variable of heat....
View Full Document

This note was uploaded on 10/05/2010 for the course BIO 101 taught by Professor Salazar during the Spring '10 term at Punjab Engineering College.

Ask a homework question - tutors are online