answers study questions 8

answers study questions 8 - Answers to study questions 8...

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Answers to study questions 8 Enzymes and metabolism You have found a test tube containing an amber-colored liquid. A very small drop of this liquid added to a solution of pyruvate in a buffer* results in the appearance in the reaction mix of acetic aldehyde and CO2. Assume that you can easily measure the quantities of reagents and products of this reaction. Your task is to determine the basis of the amber liquid properties. *(a solution that resists changes in pH used to ensure that physiological conditions are employed) 1. Does the amber solution contain a catalyst? How would you determine it? Catalysts lower the activation energy of a reaction. Enzymes are a type of catalysts that are of bi- ological origin, and are very specific. If the reaction proceeds faster with some of the amber liq- uid than without , a catalyst is present. 2. Does the amber solution contain an enzyme? How would you determine it? Enzymes are either proteins or RNA molecules. Proteins are most common. So, one could try heating the amber solution by boiling it. Most proteins would be denatured by the heat treatment and would lose their activity. An enzyme that digests RNA to nucleotides is readily available and
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This note was uploaded on 10/06/2010 for the course BIO SCI 2A 54936 taught by Professor Comai during the Spring '10 term at UC Davis.

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answers study questions 8 - Answers to study questions 8...

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