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Lab1writeup - Organic Chemistry Lab B Beatrice Nakiryowa...

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Organic Chemistry Lab B Beatrice Nakiryowa and Garth Franklin Identification, Separation, and Recovery of Fluorene and Fluorenone using Thin Layer Chromatography, Column Chromatography, and Crystallization The purpose of this lab was to become familiar with separation and purification techniques for organic compounds. Thin Layer Chromatography was used to determine whether a solution contained multiple organic compounds, to identify organic compounds in a mixture, and to learn about their relative polarities. Column chromatography was used to separate organic compounds from a mixture. Crystallization was used to purify the separated samples. Analyzing melting points was used for identification of the organic compounds. In Thin layer chromatography, 3.00g of a sand, fluorene, and fluorenone mixture was dissolved in about 28 mL of dichloromethane. The sand was removed from the solution by gravity filtration using a fluted filter paper. The filtrate was evaporated in a rotary evaporator. In thin layer chromatography, two or more compounds are distributed between a stationary and moving phase. The TLC plates were spotted with the filtrate and eluted using dichloromethane. R f values were calculated and used to identify the components of the solution. R f is calculated by dividing distance traveled by substance by distance traveled by solvent front. In week two, we used column chromatography to separate fluorene from fluorenone. A sample size of about 0.5 grams was dissolved in about 1 ml of dichloromethane and added to the column. Hexane was added to the top of the column attached to a semi-automatic pump system. Hexanes were used to elute the column and small fractions of the eluent were collected in small test tubes. The presence of organic compounds was determined using TLC plates. The fractions with positive organic compounds identification were ran through thin layer chromatography. The fractions with the same organic compounds present were combined into a round bottomed flask and evaporated. In week three, ethanol, acetone, dichloromethane, petroleum ether, and water were tested
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This note was uploaded on 10/06/2010 for the course ES ES271 taught by Professor Machaut during the Spring '10 term at Colby.

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Lab1writeup - Organic Chemistry Lab B Beatrice Nakiryowa...

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