{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

BC367PS4-1 - Brian Blanchard BC367 Biochemistry of the Cell...

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Brian Blanchard BC367 Biochemistry of the Cell I PS#4 Q1 Hydroxyapetite chromatography is a method of protein purification that harnesses the affinity of negatively and positively charged regions within a residue for opposite charges on the stationary resin of HA. HA is essentially a resin in the form of a crystalline matrix decorated with negatively charged phosphate groups and positively charged calcium ions. This method of protein purification has been used extensively for antibody purification. With the correct buffer selection, this process is very effective at separating antibodies with neutral, acidic, or basic natures. For example, HA has a very high tollerence for sodium chloride as a loading medium for acidic antibodies (allows the antibodies to remain in solution), and allows for the dissociation of ionic interactions between DNA contaminants and the desired antibodies. This method had also proven useful for binding weakly interacting basic proteins by the inclusion of 1 mM phosphate into the buffer, thereby suppressing the ability of the HA’s calcium cations to repel amines. (Gagnon, P; Frost, R; Tunón, P; Ogawa, T. 2009) Gagnon, P; Frost, R; Tunón, P; Ogawa, T . CHT Ceramic Hydroxyapetite – A New Dimesion in Chromatography of Biological Molecules . Bio-Rad Tech Note 2156 (Chromatography). Bio-Rad Laboratories, Inc., Life Science Group. 2009.
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern