Lab Report - Mole Bio 3-27-10

Lab Report - Mole Bio 3-27-10 - The objective of this...

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Rubisco is the most abundant enzyme in plants, making up 60% of soluble leaf protein. RBCS-3B encodes for ribulose bisphosphate carboxylase, an enzyme with an active site made up of eight subunits. The gene is located in the nuclear genome, where is converts carbon dioxide into organic carbon in a process called carbon fixation. In the carbon-fixing cycle, rubisco acts on ribulose biphosphate, which is used to make sucrose for food. New advancements in genetic engineering have depended on innovations to make more efficient crops. Studies have been performed on Arabidopsis thaliana to better understand the expression of the RBCS-3B gene and RBCS gene family. A study by suggested that RBCS-3B expression was affected by light exposure. One study observed that relative expression levels of four genes encoding the small subunit of rubisco was affected by different temperatures (Yoon, et. Al, 2002). Gene expression was assayed using the RACE technique and the transcript levels of four genes were compared.
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Unformatted text preview: The objective of this experiment was to determine the differences in expression of the RBCS-3B gene in Arabidopsis thaliana plants exposed to 24 hours of no light as opposed to 24 hours of light. Plants aged three weeks, four weeks, and five weeks were already grown and prepared in a 24 C growth incubator with light exposure. One plant from three, four, and five weeks of growth were taken from the growth incubator and put into a dark chamber for nineteen hours. A 0.2 gram sample of leaves from each plant were taken and frozen immediately in liquid nitrogen and stored at -20C until mRNA extraction. The interaction between phytochrome and different photoreceptors on plants is important to understanding the photoregulation of rbcS genes. Dedonder also found that the regulation of rbcS genes also depends on the developmental age of the plant....
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This note was uploaded on 10/12/2010 for the course BC BC367 taught by Professor Millard during the Spring '10 term at Colby.

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