Unformatted text preview: The objective of this experiment was to determine the differences in expression of the RBCS-3B gene in Arabidopsis thaliana plants exposed to 24 hours of no light as opposed to 24 hours of light. Plants aged three weeks, four weeks, and five weeks were already grown and prepared in a 24 C growth incubator with light exposure. One plant from three, four, and five weeks of growth were taken from the growth incubator and put into a dark chamber for nineteen hours. A 0.2 gram sample of leaves from each plant were taken and frozen immediately in liquid nitrogen and stored at -20C until mRNA extraction. The interaction between phytochrome and different photoreceptors on plants is important to understanding the photoregulation of rbcS genes. Dedonder also found that the regulation of rbcS genes also depends on the developmental age of the plant....
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This note was uploaded on 10/12/2010 for the course BC BC367 taught by Professor Millard during the Spring '10 term at Colby.
- Spring '10