exam1key

exam1key - Exam 1 BC 367 October 12, 2006 Answer Key 1. E...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Exam 1 BC 367 October 12, 2006 Answer Key 1. E 2. E 3. B 4. C 5. B 6. C 7. A 8. A 9. D 10. D 11 . A B C D E Asn-Gly-Asn His-Lys-Lys Trp- Met-Tyr Asp-Cys-Pro Val-Phe-Ala Which one of the above peptides: B elutes first from a column containing DEAE-Sephadex resin at pH 6.0 E is the most likely to be found in the center of an α helix D has the lowest pI B is the most water-soluble at pH 4.0 C elutes first from a gel filtration column C has the highest absorbance at 280 nm C is partially degraded during acid hydrolysis 12. Given the following information, briefly describe the quaternary structure of the protein. The Sanger reagent produces equimolar amounts of DNP-Val and DNP- Phe. Size-exclusion chromatography experiments indicate the protein has a molecular weight of 60 kd. Repeating these experiments in 5 M urea indicates that MW = 30 kd. When you repeat these experiments in 5 M urea plus 10 mM β -mercaptoethanol, two polypeptides emerge from the column, one with an apparent molecular weight of 10 kd and another with an apparent molecular weight of 20 kd. Two different chains; one has an Val N-term; the other has Phe.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 10/12/2010 for the course BC BC367 taught by Professor Millard during the Spring '10 term at Colby.

Page1 / 4

exam1key - Exam 1 BC 367 October 12, 2006 Answer Key 1. E...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online