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exam1key - Exam 1 BC 367 Answer Key 1 E 2 E 3 B 4 C 5 B 6 C...

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Exam 1 BC 367 October 12, 2006 Answer Key 1. E 2. E 3. B 4. C 5. B 6. C 7. A 8. A 9. D 10. D 11 . A B C D E Asn-Gly-Asn His-Lys-Lys Trp- Met-Tyr Asp-Cys-Pro Val-Phe-Ala Which one of the above peptides: B elutes first from a column containing DEAE-Sephadex resin at pH 6.0 E is the most likely to be found in the center of an α helix D has the lowest pI B is the most water-soluble at pH 4.0 C elutes first from a gel filtration column C has the highest absorbance at 280 nm C is partially degraded during acid hydrolysis 12. Given the following information, briefly describe the quaternary structure of the protein. The Sanger reagent produces equimolar amounts of DNP-Val and DNP- Phe. Size-exclusion chromatography experiments indicate the protein has a molecular weight of 60 kd. Repeating these experiments in 5 M urea indicates that MW = 30 kd. When you repeat these experiments in 5 M urea plus 10 mM β -mercaptoethanol, two polypeptides emerge from the column, one with an apparent molecular weight of 10 kd and another with an apparent molecular weight of 20 kd.
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