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Solutions2 - BC368 Biochemistry of the Cell II Problem Set...

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BC368 – Biochemistry of the Cell II Problem Set #2 – Membrane Transport ANSWERS 1. Because conductance in the presence of Ionophore X is affected so dramatically by temperature, X must be a carrier. At temperatures below the transition temperature of the membrane X cannot move across the membrane. The conductance in the presence of Ionophore Y is not affected significantly by temperature, consistent with an ionophore that works by forming a channel in the membrane. 2. It is necessary to do a double-reciprocal plot (a Lineweaver-Burk plot) of these data to assess the impact of the sodium. Based on that plot (below) it is likely that sodium is acting as a competitive inhibitor to the potassium transporter. The transporter is a carrier protein with a Vmax of about 10 μ mol/g-hours and a K t of 0.01 mM K + . 3. -4.3 kcal/mol = RT ln {[Na + ] in /[Na + ] out } + ZF ∆Ψ -4.3 kcal/mol = RT ln {[Na + ] in /[Na + ] out } + (1)F(-0.07V) at 37 o C: -4.36 = ln {[Na + ] in /260} [Na + ] in = 3.3 mM
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This note was uploaded on 10/12/2010 for the course BC BC367 taught by Professor Millard during the Spring '10 term at Colby.

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Solutions2 - BC368 Biochemistry of the Cell II Problem Set...

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