10. Bio 214 recombinant Tech

10. Bio 214 recombinant Tech - Recombinant DNA Technology...

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Recombinant DNA Technology
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Recombinant DNA technology Techniques that allows DNA to be combined from different sources DNA cloning is a technique to reproduce DNA fragments
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Model Organisms E. coli –grows rapidly (20 min generation time), easy to work with, easy to transform, normally not pathogenic and inexpensive
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Cloning a Foreign Gene into E. coli
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Cloning a Foreign Gene Into E. coli
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The First Commercially Available Human Gene Product was Insulin Cloned in 1979 by Genetech In 1992 the recombinant form of human insulin was approved
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Approach: Clone Insulin into an Expression Vector of E.coli HOW TO GENERATE INSULIN IN BACTERIA?
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Synthetic DNA oligonucleotides short pieces of DNA or RNA (2-30 nucleotides) can be synthesized to have known nucleotide sequences
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The Polymerase Chain Reaction synthesizes large quantity of a DNA fragment three steps repeated denature target DNA with heat anneal to primers specific for target DNA synthesize copies of target DNA
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Fig. 14.8
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10. Bio 214 recombinant Tech - Recombinant DNA Technology...

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