9. bio214 recombinationand gene transfer

9. bio214 recombinationand gene transfer - DNA...

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DNA Recombination and Gene Transfer
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Biological Roles for Recombination Exchange of genes between 2 DNA molecules Contributes to population’s genetic diversity
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Types of Recombination 1. Homologous - occurs between sequences that are nearly identical (e.g., during meiosis) 1. Site-Specific - occurs between sequences with a limited stretch of similarity; involves specific sites 1. Transposition – DNA element moves from one site to another, usually little sequence similarity involved
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Reciprocal General Recombination most common type of recombination a reciprocal exchange between pair of homologous chromosomes results from DNA strand breakage and reunion, leading to crossing-over
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Reciprocal general recombination
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EM of a Holiday Junction w/ a few melted base pairs around junction
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Nonreciprocal General Recombination incorporation of single strand of DNA into chromosome, forming a stretch of heteroduplex DNA proposed to occur during bacterial transformation
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This note was uploaded on 10/06/2010 for the course BIO 214 taught by Professor Dr.xinfan during the Spring '10 term at West Chester.

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9. bio214 recombinationand gene transfer - DNA...

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