PP02 - BIO 205L September 15, 2008 Laboratory Entrance Quiz...

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Unformatted text preview: BIO 205L September 15, 2008 Laboratory Entrance Quiz questions for this week will be taken from:- Chapter 3 (all) of the laboratory manual;- Today's lecture;- Information and instructions in Exercise 3 of the Lab manual. Magnification of a lens: Mag lens = length of image length of object object (100 m) image 100 m 2 mm = 0.1 mm 2 mm = 20 no units X (2 mm) recall that 1 mm = 1,000 m Mag total = Mag ocular lens X Mag objective lens Total magnification of a compound light microscope length of object length of viewed image Calculations for Determining Sizes of Objects Viewed Through a Compound Light Microscope length of object = length of viewed image Mag total Mag total = Definition: Rearrange equation: Aberration (in a light microscope) An indistinct image seen through the microscope caused, in part, by differences in the paths of light of different colors passing through a glass lens. Resolving Power (= Resolution) The closest that two objects (or edges) can be together and still distinguished as separate entities. note: resolving power is expressed quantitatively, in units of length. Object Image Magnification Exceeding the Limit of Resolution of a Photocopy Machine 1 2 3 4 The magnification of a microscope may be increased until artifacts seen in the image become noticeable and are easily confused with components of the object.with components of the object....
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PP02 - BIO 205L September 15, 2008 Laboratory Entrance Quiz...

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