PP05 - Dr. A. William Allen Office Hours: Monday 10-1 PM or...

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Dr. A. William Allen Office Hours: Monday 10-1 PM or by appointment Office: PAI 1.22G Email: billallen@mail.utexas.edu Phone: 471-2691
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To be Learned in Exercise 5 Be able to identify the parts of corn seedling. Be able to identify stages of mitosis in prepared slides of corn seedling roots. How to initiate and recognize the various developmental stages of the sea urchin. How to use a hemacytometer and serial dilutions to determine the concentration of sea urchin sperm. Describe the affect of temperature and LiCl on the development of sea urchin embryos.
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http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=416198
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Prokaryotic cell (before kernal) Type of cell lacking a membrane-enclosed nucleus Lacking membrane- enclosed organelles 1-10 μm in size
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Eukaryotic cell (true kernel) Cell type with a membrane-enclosed nucleus Membrane-enclosed organelles 10-100 μm in size
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protostomes ("first mouth") deuterostomes ("second mouth") Protostomia Deuterostomia Blastopore forms future mouth & anus Blastopore forms future anus Few HOX genes for the posterior Multiple HOX genes for the posterior Spiral cleavage of embryo Perpendicular cleavage planes in embryo Early cleavage cells committed; no identical twins Early cleavage cells totipotent; identical twins possible Coelom arises by splitting of mesoderm Coelom arises between invaginating mesoderm during gastrulation Lophotrochozoans and Ecdysozoans Echinoderms, Acorn worms, and Chordates
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http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/I/Invertebrates.html
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Development Growth Cell division (mitosis) Differentiation Senescence (aging)
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Examples of Eukaryotic Cellular Development Germinating corn seed Emphasis on the embryonic root (radicle) Developing sea urchin embryo
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Cell Division-Mitosis Interphase : Cell growth, DNA replication Prophase : Chromosomes visible, spindle forms. Metaphase : chromosomes lined along equator of cell Anaphase : Sister chomatids separate Telophase : Nuclear envelope reforms around sister nuclei, cytokinesis and cell plate formation in plant cells)
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week’s BIO 205L exercise, measures: A. fluorescence. B. optical density.
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This note was uploaded on 10/06/2010 for the course BIO 205L taught by Professor Hanson during the Fall '07 term at University of Texas at Austin.

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PP05 - Dr. A. William Allen Office Hours: Monday 10-1 PM or...

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