10/07/101Extraction and Analysis of Proteins From Cells•Preparation of Crude Cell Preparation of Crude Cell ExtractsExtracts
10/07/102Things to Learn in This Week’s Lab1.How to extract proteins from cells.1.How to make parallel dilutions from a stock solution.1.How to determine protein concentration using a standard curve.4. How to use the concentration of a dilution to calculate the concentration of the stock solution.
10/07/103Extracting Proteins From CellsWhy extract proteins from cells?Proteins control virtually all cell activities since they perform all kinds of functions:EnzymesCytoskeletal proteinsMetalloproteins Channels, PumpsG-proteinsMotor proteinsReceptorsStructural Support MoleculesStorage proteins
10/07/104Proteins are located in all membranes, the cytoplasm, and the interior of all organelles.
10/07/105Pro te ins are am azing ly dive rs e •in terms of:function, structure, chemical propertieschargeshapepH optimumsizesolubilityCofactorsThis confounds purifying a specific protein.
6What kinds of proteinwill end up in aCrude extract?
10/07/107Enzymes: globular proteinssubstrateenzymeHydrophilic: Water soluble