PP11 - GENETIC ENGINEERING OR RECOMBINANT DNA TECHNOLOGY T...

Info iconThis preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon
GENETIC ENGINEERING OR RECOMBINANT DNA TECHNOLOGY
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Thing s  to  Le a rn Fro m  This  We e k’s  La b Ho w to  us e  re s tric tio n e nzym e s  to  c ut  DNA in s pe c ific  lo c a tio ns . Ho w to  a na lyze  the  re s ults  o f yo ur  dig e s tio n o n a g a ro s e  e le c tro pho re s is  g e l. Ligation of DNA with vectors Transformation of bacterial cells with foreign DNA
Background image of page 2
Wha t a re  Re s tric tio n Enzym e s ? restriction enzyme  (o r  restriction endonuclease ) is   a n  e nzym e  tha t  c uts   do ub le -s tra nde d  DNA The enzyme makes two incisions, one through each of the double helix (i.e., each strand).
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
The term restriction comes from the fact that these enzymes were discovered in  E. coli  strains  that  appeared  to  be  restricting  the  infection  by  certain  bacteriophages . Restriction enzymes therefore are believed to be a mechanism  evolved by bacteria to resist viral attack and to help in the removal of viral  sequences. Why Does It Called Restriction Enzymes?
Background image of page 4
Each restriction enzyme   recognizes   a   specific sequence   of nucleotides in a DNA strand . …T G A C C T   G A A T T C   A T C A T G G …   …A C T G G A   C T T A A G   T A G T A C C … Restriction     Enzyme
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Types of restriction enzymes Restriction enzymes are classified biochemically  into three types: Type I, Type II, Type III. Type II restriction enzymes are widely used in research laboratories
Background image of page 6
Image of page 7
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 10/06/2010 for the course BIO 205L taught by Professor Hanson during the Fall '07 term at University of Texas.

Page1 / 34

PP11 - GENETIC ENGINEERING OR RECOMBINANT DNA TECHNOLOGY T...

This preview shows document pages 1 - 7. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online