BB lecture 11-7 DNA replication

BB lecture 11-7 DNA replication - THE FLOW OF GENETIC...

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THE FLOW OF GENETIC INFORMATION DNA RNA PROTEIN DNA 1 2 3 1. REPLICATION (DNA SYNTHESIS)
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Chapter 16 (pp.299-307) DNA – replication Learning objectives • Know the structure of a DNA strand and how nucleotides are added during replication. • Explain how the Meselson-Stahl experiment reveals the semi-conservative pattern of replication of double stranded DNA. • Describe the steps of the replication process and the enzymes/proteins involved. • Know the source of energy for DNA synthesis. • Explain how the growth of the “leading” strand differs from that of the “lagging” strand during DNA synthesis. • Contrast the replication of DNA in prokaryotes and eukaryotes. • Be able to explain the significance of telomeres and the enzyme telomerase.
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Figure 16.8 Base pairing in DNA H N H O CH 3 N N O N N N N H Sugar Sugar Adenine (A) Thymine (T) N N N N Sugar O H N H N H N O H H N Sugar Guanine (G) Cytosine (C)
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Figure 16.5 The structure of a DNA strand Sugar-phosphate backbone Nitrogenous bases 5 end O O P O CH 2 5 4 O H H O H H H 3 1 H O CH 3 N O N H Thymine (T) O O P O O CH 2 H H O H H H H N N N H N H H Adenine (A) O O P O O CH 2 H H O H H H H H H H N N N O Cytosine (C) O O P O CH 2 5 4 O H O H H 3 1 OH 2 H N N N H O N N H H H H Sugar (deoxyribose) 3 end Phosphate Guanine (G) DNA nucleotide 2 N
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Figure 16.7 DNA STRUCTURE O O O OH P H 2 C O O O O P H 2 C O O O O P H 2 C O O O O P O OH O O O T A C G C A T O O O O OH CH 2 O O O O P CH 2 O O O O P CH 2 O O O O P CH 2 O O O O P 5 end Hydrogen bond 3 end 5 end 3 end C T A A T C G G C A T C G A T A T A T T A C T A 0.34 nm 3.4 nm (a) Key features of DNA structure (b) Partial chemical structure (c) Space-filling model G 1 nm G H 2 C G Note types of chemical bonding & where
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In an analysis of the nucleotide composition of DNA, which of the following is true ? a. A = C b. A = G and C = T c. A + C = G + T d. A + T = G + C e. Both B and C are true
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A nucleic acid was analyzed and found to contain 32 percent A, 18 percent G, 17 percent C, and 33 percent T. The nucleic acid must be: a.single-stranded RNA b.single-stranded DNA c.double-stranded RNA d.double-stranded DNA
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A nucleic acid was analyzed and found to contain 37 percent A, 16 percent G, 22 percent C, and 25 percent T. The nucleic acid must be: a.single-stranded RNA b.single-stranded DNA c.double-stranded RNA d.double-stranded DNA
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Conservative model. The two parental strands reassociate after acting as templates for new strands,
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This note was uploaded on 10/06/2010 for the course BIO 311C taught by Professor Satasivian during the Fall '08 term at University of Texas.

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BB lecture 11-7 DNA replication - THE FLOW OF GENETIC...

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