W2010ExamIAnswer

W2010ExamIAnswer - Exam I: Form A 153L Winter 2010 Name:_...

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Exam I : Form A Name:_____________________ 153L Winter 2010 TA:_________Section:______ Chem 153L, Winter 2010 Exam I February 2, 2010 TA Use Only: Be sure to: Write your name on each page. Check that there are 6 pages including the cover page. Show all work – for math problems, if strategy is not shown , then points will not be awarded! Do not skip logical steps in explaining your reasoning. Keep answers direct. Do not repeat the question in your answer. Run-on sentences will be grammatically corrected & then graded based on the new sentence count. “Shotgun” answers (i.e. full of keywords in a nonsensical fashion) can receive zero credit. If you don’t know the answer to a question, then write what you think – don’t try to guess. Multiple choice could have more than one right answer. Wrong answer(s) result(s) in deductions. Score: Q.______ /20 pts. E1.______E2.______ E3________= _______ / 80pts.
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Exam I : Form A Name:_____________________ 153L Winter 2010 TA:_________Section:______ LDH LAB 1 Questions 1. List the factors that affect the final volume of the crude extract. Phosphate buffer, meat tissue, transfer loss, removal of insoluble cellular debris. (+4, -1 for any missing item up to 4 pts.) __________________________________________________________________________ 2. How will the volume of the 40% ammonium sulfate supernatant relatively compare to the crude extract (lesser, greater, or same)? Explain your answer. Greater. The amount of salt is greater than the amount of precipitating cellular molecules. (+2, NO points awarded without proper explanation) 3. Which reagents are required to test the “no pyruvate” control? What dilution factor of the crude extract will provide the best comparison to your test samples? Buffer, crude extract, and NADH solution (+1). Optimal dilution factor for the enzyme assay. (+1) 4. Which steps are required in preparing the crude extract? Circle the letter to all that apply. a) Use cold temperatures: cold buffer, cut tissue on ice, use chilled blender cups, centrifuge at 4 o C. b) Add a concentration of SDS lower than the CMC to lyse cells and thus subjecting the sample to less thermal energy. c) Reduce thermal energy by blending the sample in 30s bursts, and waiting 30s before you perform the next 30s burst. d) After centrifugation, resuspend the pellet in cold buffer, and filter through glasswool to remove insoluble contaminants. (+2) Correct: A, C. Incorrect: B is wrong because SDS is not used since we know that at the very least it will interfere with our protein assay. D is wrong because we discard the crude pellet as it contains dense insoluble material.
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Exam I : Form A Name:_____________________ 153L Winter 2010 TA:_________Section:______ 5. Which steps are required in preparing the 40% ammonium sulfate step? Circle the letter to all that apply. a) After centrifugation of the 40% step, resuspend the pellet in cold buffer, and remove an
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This note was uploaded on 10/07/2010 for the course POLI SCI 40 taught by Professor Sch during the Spring '10 term at UCLA.

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W2010ExamIAnswer - Exam I: Form A 153L Winter 2010 Name:_...

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