Lecture13-15Summer10BW

Lecture13-15Summer10BW - Kinetics! Relates [Reactant] to...

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1 Kinetics Relates [Reactant] to Rate of Change Other conditions kept constant such as T, P, etc. Vary only [Reactant] Obtain rate constant or k = rate/[Reactant] Chemical Kinetics No Catalyst for reaction Adjust conditions such as T, P, Solvents Usually conditions cannot sustain life. So conditions are catalysts (no enzymes) Measure in diff. ways. Use k to predict rates One k per step. InFnite Relationship Enzyme Kinetics Catalyst in Reaction Only one substrate is varied. Constant: T, [other substrate], [enzyme] [Enzyme] Constant – limit to rate increase.
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2 MM Theory – Simplifed Enzyme Reaction Make our reaction consistent by experimental design One substrate in excess and constant. Initial velocity – no reverse reaction. Recall – How do we measure rate? In our lab? Abs/change oF NADH, Only happens ES P Assay Design • On substrate is constant and excess. – Ideally the one being measured so that
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This note was uploaded on 10/07/2010 for the course POLI SCI 40 taught by Professor Sch during the Spring '10 term at UCLA.

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Lecture13-15Summer10BW - Kinetics! Relates [Reactant] to...

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