ExamIFormAAnswerSummer2010 - Exam I: Form A 153L SUMMER...

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Exam I : Form A Name:_____________________ 153L SUMMER 2010 TA:_________Section:______ Chem 153L, SUMMER 2010 Exam I July 12, 2010 TA Use Only: Be sure to: Write your name on each page. Check that there are [5] pages including the cover page. Show all work – for math problems, if strategy is not shown , then points will not be awarded! Do not skip logical steps in explaining your reasoning. Keep answers direct. Do not repeat the question in your answer. Run-on sentences will be grammatically corrected & then graded based on the new sentence count. Write only in the space provided. An additional clause going over the given space provided may be allowed if necessary. “Shotgun” answers (i.e. full of keywords in a nonsensical fashion) can receive zero credit. Multiple choice questions could have more than one right answer. Wrong answer(s) result(s) in deductions. Score: E1.______E2.______ E3_______ E4_______ E5________= _______ / 80pts.
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1. (+4) Your LDH research group will be divided into two sub-groups: A (purification) and B (Assays). Circle all that is true about the LDH LAB 1 duties of the two groups. a) Group A need not know any of the procedural steps or anticipated results of Group B. b) Group B must have substrate mixtures and a dilution strategy prepared as soon as Group A has completed each step. c) Group B must have BSA standards, dye, and a dilution strategy prepared as soon as Group A has completed each step. d) Group A must never assist in any of the assays because they will be switching duties next week. e) Both groups must exchange contact information, and that each member must have a copy of all the data before they leave the lab. Correct Answers: b, e. Incorrect Answers: a, c, d (+4 total, +2 for each correct. -2 for each incorrect. Deductions not exceed worth of question) 2. (+2) What is the characteristic of the contaminants that are removed during crude extract preparation? Insoluble material. (+2) 3. (+3) Why are membranes and organelles able to pellet out of solution during centrifugation as opposed to proteins? Explain your reasoning based on chemical interactions and the theory of centrifugation. They contain a higher proportion of nonpolar interactions (+1) which are more dense (more mass within an unit volume due to shorter bonds) than polar interactions (+1) and thus are able to displace the solvent below the solute upon centrifugation. (+1) 4. (+4) Which is true with regards to the ammonium sulfate steps? (Circle all that apply) a) The steps are measured by percent saturations which are quantified by mass of salt in the total volume of the final solution.
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This note was uploaded on 10/07/2010 for the course POLI SCI 40 taught by Professor Sch during the Spring '10 term at UCLA.

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ExamIFormAAnswerSummer2010 - Exam I: Form A 153L SUMMER...

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