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Fall09ExamIAnswer - Exam I: Form A 153L FALL 2009...

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Exam I : Form A Name:___ANSWERS________ 153L FALL 2009 TA:_________Section:______ Chem 153L, FALL 2009 Exam I Cover Page TA Use Only: Be sure to: Write your name on each page. Check that there are 5 pages including the cover page. Show all work – for math problems, if strategy is not shown , then points will not be awarded! Do not skip logical steps in explaining your reasoning. But keep the answers direct. Do not repeat the question in your answer. Run-on sentences will be grammatically corrected & then graded based new sentence count. “Shotgun” answers full of keywords that do not make sense will not result in full credit. If you don’t know the answer to a question, then write what you think – don’t try to guess. Multiple choice – could have more than one right answer. Wrong answers results in deductions. Score: P1.______P2.______P3.______ P4________= _______ / 80pts.
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Exam I : Form A Name:___ANSWERS________ 153L FALL 2009 TA:_________Section:______ 1. The following are various steps that are involved in the events that lead up to the interaction between the protein and commassie blue. They are not in chronological order. (a) The negative charge of the dye and positive charge of the proteins form an ionic bridge. (b) Given a sufficient time, there presumably no net change in the bound versus unbound. (c) As the coomassie red form loses protons, the probability for the unprotonated forms to be protonated decreases. (d) The ionizable groups of the protein become protonated. (e) Nonpolar regions of the dye and the hydrophobic pockets of the protein for[m] stable Van der waals interactions. (f) The protein denatures. (g) Peptide bonds begin to break. Using the above list, provide the order of steps that ultimately lead up to protein binding to dye in the space provided: __ d, f, e, a, c, b, g __(7 pts. -1 for each out of order)________ 2. Suppose Shuktika used deionized water when making all her dilutions for her unknowns during this week’s lab. Mikhail made a repeat set of the same unknowns using buffer. Suppose they ran separate enzyme and protein assays. When comparing their results they found: (Circle all that apply) (a) Enzyme activities were the same because they only measure the initial velocity. (b) Concentrations of protein were the same because water only helps the unfolding of the protein because deionized water has a pH of 8 to 9. (c) Concentration of proteins w[as] the same because water only helps the unfolding of the protein because deionized water has more excess protons than buffer. (d) Mikhail had a higher enzyme yield than Shuktika for the 40% sup because the effects of water in Shuktika’s experiments were systematic. (e) Mikhail had higher specific activities than Shuktika because the effects of water in
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This note was uploaded on 10/07/2010 for the course POLI SCI 40 taught by Professor Sch during the Spring '10 term at UCLA.

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Fall09ExamIAnswer - Exam I: Form A 153L FALL 2009...

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