Q3AAnswerVOHSpring2010

Q3AAnswerVOHSpring2010 - CHEM 153L QUIZ 3 FORM A Tuesday,...

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CHEM 153L QUIZ 3 FORM A Tuesday, May 4, 2010 NAME: ____ANSWER KEY______ SECTION: ____________ TA use only: Page totals = P1. _______ P2 ________ P3 _________ = ___________ General Instructions: 1. Answers written in with pencil or erasable pen may forfeit any possible regrades. 2. If you have finished early, place your quiz face down and wait till time is called. Do not leave your seat until the quiz is done. 3. Unless specified, multiple-choice questions may have more than 1 correct answer. Circle all that apply. If you select the wrong choice, you will be deducted points – so avoid guessing. READ EACH SELECTION CAREFULLY. 4. It is preferred that you limit your answers to the space provided unless instructed otherwise. If you need to go over, do not exceed more than a sentence. 5. Gauge your time. Give yourself about 8 minutes per page. The quiz is 30 minutes.
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1. During the start of LDH LAB 2, Group A will do which of the following steps? (Circle all that apply) a) Centrifuge the resuspended 60% pellet in the clinical centrifuge in order to remove undissolvable material. b) Centrifuge the resuspended 60% pellet in the clinical centrifuge in order to precipitate out LDH and proteins of equal solubility. c) Resuspend the 60% pellet using pH 7.2 buffer. d) Run all enzyme and protein assays on the 60% supernatant and determine recovery so that Group B can proceed with the affinity column. e) After removing the aliquot for assaying, leave the resuspended 60% pellet out at room temperature before giving it to Group B. Correct Answer: A, E. (+2) Deduct 1 pt for every wrong answer, not to exceed pts of question. C is wrong because you are to resuspend in a pH 6.0 buffer which increases the protonation state of LDH and thus increases binding to AMP and it’s negative charges and dipoles. D is wrong because protein assays are not required to move forward to the affinity column step and should be reserved till after the column is completed. Remember that you want all purification steps so you can efficiently run one standard
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Q3AAnswerVOHSpring2010 - CHEM 153L QUIZ 3 FORM A Tuesday,...

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