Spring2010ExamIFormABlank

Spring2010ExamIFormABlank - Exam I Form A 153L SPRING 2010...

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Exam I : Form A Name:_____________________ 153L SPRING 2010 TA:_________Section:______ Chem 153L, SPRING 2010 Exam I April 27, 2010 TA Use Only: Be sure to: Write your name on each page. Check that there are 6 pages including the cover page. Show all work – for math problems, if strategy is not shown , then points will not be awarded! Do not skip logical steps in explaining your reasoning. Keep answers direct. Do not repeat the question in your answer. Run-on sentences will be grammatically corrected & then graded based on the new sentence count. “Shotgun” answers (i.e. full of keywords in a nonsensical fashion) can receive zero credit. Multiple choice questions could have more than one right answer. Wrong answer(s) result(s) in deductions. Score: E1.______E2.______ E3_______ E4_______ E5________= _______ / 80pts.
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1. In the space provided below, list the three main steps that we will employ to prepare our crude extract and the basic purpose of each step. 1 st Step: __ 2 nd Step: __ 3 rd Step: __ 2. How will you use the volumes of each purification step during LDH LAB 1? (Circle all that apply) a) Qualitatively monitor the progress of your steps. b) Use total volumes of each step, which include the test aliquot, for the purification table. c) Determine the optimal dilution factor of 40% (NH 4 ) 2 SO 4 step enzyme assays. d) Use total volume of each step, which include the test aliquot, for (NH 4 ) 2 SO 4 steps. e) Determine the optimal dilution factor of the crude enzyme assays. 3. Which is true with regards to the “no pyruvate” control? (Circle all that apply) a) No activity will precisely confirm that LDH is the only enzyme in the crude that reacts with NADH and pyruvate. b) The control consists of only 100 µ l of crude extract and 0.500 ml of 0.9 mM NADH. c) Measurable once optimal enzymatic data of the crude extract has been obtained. d) Assuming no activity, the starting absorbance is exactly the same as the no enzyme control. e) Even with no activity when testing the control with the crude extract, the protocol dictates that this control is necessary for every subsequent purification step. 4. Suppose you carry out a 50% ammonium sulfate precipitation as part of the 1
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This note was uploaded on 10/07/2010 for the course POLI SCI 40 taught by Professor Sch during the Spring '10 term at UCLA.

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Spring2010ExamIFormABlank - Exam I Form A 153L SPRING 2010...

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