Lecture 101 RNA and Protein Chiu 2008

Lecture 101 RNA and Protein Chiu 2008 - Chi- hua Chiu Dept....

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Unformatted text preview: Chi- hua Chiu Dept. of Genetics Office Hours: Tuesdays, 12:45-1:45 Douglass Campus MSLC No Office hours Tuesday November 25. Copy of important figures (not in your book) and lecture guide will be on reserve at the MSLC. RNA and Protein Synthesis Chapter 13 DNA Transcription RNA Translation Protein The Central Dogma coupled in prokaryotes decoupled in eukaryotes Figure 13-4 Transcription Synthesis of RNA using a DNA template RNA is synthesized from DNA template Single stranded A, G, C, U (not T) Messenger RNA (mRNA) Ribosomal RNA (rRNA) Transfer RNA (tRNA) RNA types RNA polymerase II: (mRNA) RNA polymerase I: (rRNA) RNA polymerase III: (tRNA) Enzymes involved in Transcription-usually single stranded-uracil is substituted for thymine-sugar is ribose-phosphodiester bond links nucleotides together RNA structure Fig. 13-3 5 - ATGGCCTGGACTTCA - 3 Sense strand of DNA 5 - ATGGCCTGGACTTCA - 3 Sense strand of DNA 3 TACCGGACCTGAAGT 5 antisense strand of DNA 5 - ATGGCCTGGACTTCA - 3 Sense strand of DNA 3 TACCGGACCTGAAGT 5 antisense strand of DNA (template strand) DNA directed RNA polymerases also carry out synthesis in the 5 3 direction. 5 - ATGGCCTGGACTTCA - 3 Sense strand of DNA 3 TACCGGACCTGAAGT 5 antisense strand of DNA (template strand) Transcription of template strand 5 AUGGCCUGGACUUCA 3 mRNA (messenger RNA) Growing RNA strand Template DNA strand 5 end 3 Nucleotide added to growing chain by RNA polymerase 3end 5 Fig. 13-7 5 3 Non-coding promoter DNA Non-coding enhancer DNA Can control transcription (generation of RNA) transcription 3 5 5 3 Primary mRNA transcript Eukaryote gene structure split genes protein coding regions are interrupted by non-coding regions Introns DNA sequences that will not be translated. They do not encode information that corresponds to amino acids. These are spliced out of the messenger RNA during post-transcriptional processing. Exons DNA sequences that will be translated. They encode information that leads to formation of a corresponding string of amino acids (i.e. the protein product). 5 3 DNA GT AG GT AG 5 regulatory region Transcription 3 Pre-mRNA GT AG GT AG 5 5 UTR 3 UTR Processed mRNA Exon 1 Exon 2 Exon 3 5 3 Translation Polypeptide N-terminus C-terminus 5 3 Primary mRNA transcript contains both exon and intron sequence Figure 13-9 Post-transcriptional processing of primary messenger RNA Introns spliced out other modifications to mRNA 5...
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Lecture 101 RNA and Protein Chiu 2008 - Chi- hua Chiu Dept....

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