Ex1-2 Culture Media Preparation Lab lecture(3)

Ex1-2 Culture Media Preparation Lab lecture(3) - 9/10/10...

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9/10/10 1 Experiment 1-2: ((pgs. 14-17) Culture Media Preparation/ Complex and Defned Media (Handout) Purpose : To learn the process of making media and the important factors needed for media. Media : food that is used for culturing bacteria and other microorganisms Three Consistencies oF Media: 1. Liquid 2. Solid 1.5% Agar concentration 3. Semisolid 0.4 ~ 0.5% Agar concentration Solid and semisolid media are made by adding varying concentrations of a solidifying agent. A good solidifying agent : 1. Is not utilized by the microorganisms 2. Doesn’t inhibit bacterial growth 3. Doesn’t liquefy at room temperature Gelatin is sometimes used as a solidifying agent, but is is hydrolyzed by many organisms and liqueFes at room temperature. Agar (made from seaweed) and silica gel are good choices for solidifying agents. Nutrients : chemical compounds that can be broken down into or used to make monomers macronutrients : nutrients required in large amounts: ex. C, N, P, S, K, Mg, Ca, Na, ±e micronutrients : nutrients required in small amounts: ex. Metals Nutritional Requirements : 1. water – all organisms are ~ 75% water - solvent for all chemical reactions - provides an environment for molecular movement 2. carbon – provides a source of carbon - autotrophs – can utilize the carbon in CO 2 to synthesize all materials - heterotrophs – must have one or more organic compounds for carbon
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Ex1-2 Culture Media Preparation Lab lecture(3) - 9/10/10...

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