BIO201 - Lecture 30

BIO201 - Lecture 30 - Lecture #30. 4-4-08 How does DNA...

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Lecture #30. 4-4-08
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How does DNA replicate? Note: This is not transcription. How can you tell it is replication and not transcription? A is paired with T
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Replication of bacterial, circular DNA Is this dispersive, conservative or semi-conservative? Semi-conservative
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DNA Replication in Eukaryotes Can’t use gradient density centrifugation to separate chromatids or DNA from eukaryotes Use metabolically labeled DNA and look at mitotic chromosomes 1. Must use mitotic cells to see compacted chromatids 2. Use BrdU (bromodeoxyuridine) to substitute for thymidine in DNA A:T A:BrdU 3. Thymidine stains dark. BrdU doesn’t. 4. All T is dark, all BrdU is light, hybrid is dark
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In Eukaryotes Note: All T is dark, all BrdU is light, hybrid is dark
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In Eukaryotes Note: All T is dark, all BrdU is light, hybrid is dark
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In Eukaryotes Note: All T is dark, all BrdU is light, hybrid is dark
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Dark Both chromatids dark Light (BrdU) Dark No BrdU – dark Hybrid – dark All BrdU - light BrdU is red, light
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BrdU Hybrid In Second generation
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BIO201 - Lecture 30 - Lecture #30. 4-4-08 How does DNA...

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