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Unformatted text preview: the wells to determine the sizes of the fragments. Material and Methods Measured Agarose powder and added to a flask. Added 100 ml TAE Buffer to the flask. the agarose was heated in microwave to make into solution. the solution was coole to about 70C,. Place the combs in the gel casting tray. The melted agarose solution was placed in casting tray and let cool.the combs were carefully pulled out. The gel was placed in the electrophoresis chamber. TAE Buffer was added so that there was about 2-3 mm of buffer over the gel. Loading the gel Works Cited Nucleic Acid Extraction 3 gDNA. (2010). Wikipedia . Retrieved (2010, September 15) from http://en.wikipedia.org/wiki/GDNA Polymerase chain reaction. (2010). Wikipedia . Retrieved (2010, September 15) from http://en.wikipedia.org/wiki/PCR...
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