Lab 5 gel2 - the wells to determine the sizes of the...

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Nucleic Acid Extraction 1 Nucleic Acid Extraction Giannina Morales Temitope Foleyson BIO 213L 1 9-15-10
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Nucleic Acid Extraction 2 Introduction Nested PCR is a second round pcr modifying the polymerase chain reaction intended to reduce contaminants in products due to unexpected primer binding sites.Nested PCR uses two reaction primers. First primer binds to sequences outside of dna and the second primer attatches and increases targets within the products of first reaction.Gel electrophoresis is a technique used for the separation of Dna Rna or protein molecules using an electrical force applied to the agarose gel. the phosphate backbone of the DNA - charge of phosphate is attracted to to the positive charge of the voltage of the machine. the agarose gel, depending on the concentration, will have little holes in it which the DNA travels through. smaller fragments travel faster and farther, the larger fragments travel slower and less distance. you can measure the distance from
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Unformatted text preview: the wells to determine the sizes of the fragments. Material and Methods Measured Agarose powder and added to a flask. Added 100 ml TAE Buffer to the flask. the agarose was heated in microwave to make into solution. the solution was coole to about 70C,. Place the combs in the gel casting tray. The melted agarose solution was placed in casting tray and let cool.the combs were carefully pulled out. The gel was placed in the electrophoresis chamber. TAE Buffer was added so that there was about 2-3 mm of buffer over the gel. Loading the gel Works Cited Nucleic Acid Extraction 3 gDNA. (2010). Wikipedia . Retrieved (2010, September 15) from http://en.wikipedia.org/wiki/GDNA Polymerase chain reaction. (2010). Wikipedia . Retrieved (2010, September 15) from http://en.wikipedia.org/wiki/PCR...
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Lab 5 gel2 - the wells to determine the sizes of the...

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