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Report 1 - 1. (5pt) What are the advantages of using...

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1. (5pt) What are the advantages of using immunofluorescent staining versus immunoprecipitation if you want to understand the co-localization of two proteins in the cells? (no more than 5 sentences) Immunoprecipitation confirms protein interactions through whole cell extracts and the formation of protein to protein complexes. Because immunoprecipitation requires the preparation of cell extracts, the physiological conditions under which proteins may interact in true cellular environment. In immunofluorescent staining, one would use specific antibodies coupled to different fluorophores to stain the proteins. The stain not only allows visualization of any colocalization but also a more specific location. Thus, clearly show the co-localization of two proteins in a cell. http://www.promega.com/guides/protein.interactions_guide/chap4.pdf 2. (10pt) You received two antibodies (antibody A and antibody B) against CD63 from a collaborator, but you aren’t sure which epitope on the protein the antibodies respond to. You
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This note was uploaded on 10/11/2010 for the course MCDB 165A taught by Professor Iruela-arispe during the Fall '08 term at UCLA.

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Report 1 - 1. (5pt) What are the advantages of using...

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