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Unformatted text preview: Pictu re 3 4 Alice Shum Question #1: (10 points) Tom is a molecular biology graduate student who needs to isolate a gene from the pathogenic bacteria Salmonella typhi, which he is studying. He wisely decides to use PCR to amplify the gene before trying to purify it. The gene has the general structure shown below: ~1000 Base Pairs 5’-ATGGCTTACGTG-----------CCATAGGGCTAA-3’ 3’-TACCGAATGCAC-----------GGTATCCCGATT-5’ The gene is about 1000 nucleotide base pairs long, but only the sequences at the ends of the double strand are shown. Use the information from the video, what you learned about base pairing in class, and the fact that PCR always proceeds along the parent DNA strand the 3’ to 5’ direction to design 6 nucleotide long PCR primers for Tom. Maximum number of words: 100 5'-ATGGCT-3' 3'-TTAGCC-5' Question #2: (5 points) Tom needs enough DNA from his PCR for several different analytical tests. If Tom starts with only 2 strands of DNA (1 double stranded helix) how many strands will he have after 6 rounds of PCR (Both Target DNA and Alice Shum...
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This note was uploaded on 09/21/2010 for the course ALI 231 taught by Professor Staff during the Fall '08 term at USC.
- Fall '08