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Unformatted text preview: PHCY 6101-LAB Fall 2010 LABORATORY 1
INTRODUCTION TO LABORATORY
Safety in the laboratory
• • • Please take 5 minutes and review the safety rules listed below. The instructor will show the location of and demonstrate the use of safety supplies within the laboratory. Clarification on UW Safety Office requirement for use of lab jacket, goggles, gloves, masks. Safety Rules
The School of Pharmacy and the University of Wyoming are committed to provide to all students a safe, clean and efficient environment in which to learn and work. This document describes the hazards to which students might be exposed during laboratory exercises and provides operational procedures to safeguard the students. 1. A copy of the UW Environmental Health & Safety Manual can be obtained through http://www.uwyo.edu/safety/ click on Safety Manual pdf. The Environmental Health and Safety Manual is the official document for dealing with health and safety of students and employees at the University of Wyoming. 2. There will be an official introduction to lab safety rules, use of equipment, and discussion on general techniques that will be used during labs. 3. The laboratory is equipped with all required safety devices (eyewash fountains/bottles, hand wash sink, fire extinguishers and waste disposal containers for glass and sharps). 4. Appropriate disinfectant solutions, paper towels, a dustpan, broom and squeegee are available for spills of non-infectious/non-caustic materials. All clean-up procedures will be performed by instructor or TA. LAB 1: Intro & Check-in 1 PHCY 6101-LAB Fall 2010 5. Lab coat (clean!) and eye protection must be used at all times during laboratory exercises. Additionally, whenever necessary, the use of masks and disposable gloves (provided by the School of Pharmacy) will be suggested by the instructor. 6. There is a pre-lab lecture before all laboratory exercises devoted to instruction about safe handling, use and techniques to follow. 7. Chemicals used during the laboratories are mostly salts, weak acids or weak bases, or very dilute solutions of drugs, regularly used in humans and exposition to chemical hazards extremely reduced and contamination very unlikely. Thus, the use of fume hoods is not necessary. However, all chemicals may pose unknown hazards and should be used with caution. Safe handling methods which are in keeping with good laboratory practice are expected. 8. Hand washing is highly recommended and emphasized whenever the hands get in contact with any of these agents. 9. Biologicals, blood and blood products, and infectious materials (human body fluids, human tissue) will not be handled in this lab. 10. According to the concept of universal precautions by OSHA (Occupational Safety and Health Administration), all human blood and body fluids should be assumed to be contaminated with Hepatitis B, HIV and/or other blood-borne pathogens. However, risk of exposure to bloodborne pathogen during this class is basically inexistent. If any accidental cuts or abrasions happen, the student will stop the procedure and, with the help of instructor, clean injured area and use recommended first aid measures. First aid kits for this class are placed nearby all sinks in Rm. 381(these will not be used by this class) and in a cabinet above hand washing sink in Rm. 379. If bleeding is unstoppable, the student will be directed to the Student Health Building. Students should not help with cleanup and first aid measures on another student to avoid contact with anyone else’s blood or blood-contaminated material. Only the instructor is in charge of first aid measures in the lab. 11. Splashing of any materials to face, eyes, mouth, etc, should be immediately washed with tap water and the eyewash if necessary. 12. Containers for glass disposal and puncture-resistant containers for sharps are available in the lab. 13. Eating, drinking, smoking, applying lip balm, handling contact lenses, etc, are prohibited in any room of the Pharmaceutical Care Learning Center, including pre-lab room and conference room.
• These safety rules are part of the PHCY 6101 course packet for Dose Form laboratory and the instructor expects all students to follow them accordingly. LAB 1: Intro & Check-in 2 PHCY 6101-LAB Fall 2010 Weighing and Maintenance of balances and stir/hot plates
• • • • • Each student will be issued a stir/hot plate and a pharmaceutical grade torsion balance (numbered), which must be kept clean at all times. No chemicals should be left on top of pans of balance and top of stir plates to prevent corrosion and smoke. Both pieces of equipment will be shared by two other students registered in other sections of this lab. Numbered boxes of weights (for torsion balance) and small forceps (to handle balance weights) are available in a labeled drawer in the lab center island. Weights must be kept in box to prevent losing. Seven (7) electronic balances, labeled A through F, are available to be used during most labs (determined by instructor) and a list of students responsible to clean them during each lab is posted in the pre-lab room and in the labnext to the key cabinet. Balance cleaning must be followed and are part of the lab grade for those students assigned. During Lab 2 the instructor will demonstrate basic principles related to the lab’s electronic balances and how to use and clean them. Most weighing will be performed using weighing paper unless weighing boats are recommended by the instructor (e.g. sticky chemicals, large amounts, etc). Hand washing test
Hand washing can prevent personnel-transmitted infections. Before compounding, personnel should scrub hands and arms to the elbows with an appropriate cleanser. Hand washing is sometimes incorrectly omitted when gloves are worn, the assumption being that they provide enough protection. In fact, microorganisms multiply rapidly inside warm moist gloves and then can leak through them. The leakage rate is more than 50% when gloves are stressed during use. For this reason, the Centers for Disease Control and Prevention (CDC) and the USP have stated that gloving does not replace hand washing and that hand washing is imperative after gloves are removed. Compliance with hand washing by medical personnel is multifaceted. It is affected by individual motivation, knowledge of hand washing importance, staffing, location of sinks, supply of paper towels/dryers, conditions of employee's hands, and general enforcement policies. Ironically, it is considered the single most important procedure for preventing nosocomial infections (infections originating in a hospital or developing among hospital staff). Hand washing test: the instructor will distribute some drops of GloGerm® product into one hand of each student. The students will be asked to rub it into the other hand including on top of hands. With the help of water, soap and sponge/brush, students will try to remove as best as possible all bright orange color from their hands. Hands must be dried with paper towels before testing the hand washing quality with the UV lamp. LAB 1: Intro & Check-in 3 PHCY 6101-LAB Fall 2010 Suggested hand washing procedure for all healthcare personnel and people handling food: 1. 2. 3. 4. Remove all jewelry, such as rings and watches Wet hands and forearms thoroughly Scrub hands vigorously with an antibacterial soap Work soap under the fingernails by rubbing them against the palm of the other hand 5. Interlace the fingers and scrub the spaces between the fingers 6. Wash wrists and arms up to the elbows 7. Thoroughly rinse the soap from hands and arms 8. Dry hands and forearms using a non-shedding paper towel 9. Use a dry paper towel to turn off the water faucet 10. After hands are washed, avoid touching clothes, face, hair, or any other potentially contaminated object in the area. Washing/cleaning supplies
• • • • • • Use a piece of colored tape to write your name and apply it to your goggles/eye protector (for return in case you leave it out of your drawer). Using sponge, brush and SOS pad wash well with soap and hot water all beakers, cylinders, glass rod, mortars and pestles, spatulas and evaporation porcelain casserole. Dry with paper towel. Shake cylinders to remove the majority of the water. Wash all glassware also in the same manner after each laboratory exercise so you will be ready for the following lab. Place a piece of “butcher’s” paper as a lining on drawer and locker to keep glassware clean. Keep your eye protector in a zip bag so it doesn’t get scratched. Keep your lab coat clean so you look “presentable” and professional. If you take your coat home to wash and forget it, please let the instructor know before the beginning of the lab so a coat can be provided to you temporarily for that lab. In accordance to UW safety regulations no student will be allowed to participate in lab exercises without appropriate garb (coat and eye protector). LAB 1: Intro & Check-in 4 ...
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This note was uploaded on 10/15/2010 for the course PHCY 6101 taught by Professor Teixeira during the Fall '10 term at Pima CC.
- Fall '10