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5’ Initation: origin of replication 3’ 5’ 3’ 5’ 5’ 3’ Direction of new synthesis Helicase SSB’s Direction of new synthesis 3’ Replication Fork Unwind, Prime, & Synthesize DNA: Linear chromosomes (eukaryotic DNA): multiple origins of replication (called ori’s) Circular DNA (bacteria): single ori Note the parental & newly made DNA are anti- parallel to each other. Also, synthesis of DNA pro- ceeds in opposite directions. DNA Polymerase DNA Primase Primase RNA Primer RNA primer ATPs, GTPs, CTPs, TTP’s A pool of deoxy- nucleotides that DNA polymerase uses to synthesize DNA complimentary to parent strand. DNA Polymerase DNA Polymerase
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Discontinuous synthesis RNA primers Primase
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