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Unformatted text preview: gression for Column C Concentration of Cysteine (mM) Absorbance Sec# Group# Activity 2B Baseline Experiment Protocol Error: Please note that enzyme is 0.5 ug/ml not 5 ug/ml. Table 4.1 A 1 mg/ml stock is diluted 2000 fold for use in the assay. Reaction Tubes Control Experimental 0.1 M PB7 3.6 ml 3.5 ml Use the control tube to blank the machine 7.5 mM ATCh Use micropipettes to add reagents 5 mM DTNB Total volume 4 ml 4 ml Abs. value expected AChE Activity: Baseline Experiment Reaction conditions Time (sec) A412 nm mM thiocholine Clock Time: 30 temp: 60 90 120 150 180 pH: 210 240 270 300 Group Members: Baseline Experiment Notes: 200 l 200 l 200 l 200 l 0.5 ug/ ml AChE (enzyme) 0 l 100 l Absorbance of Control tube at end of the reaction =...
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This note was uploaded on 10/18/2010 for the course BIO 204 taught by Professor O'neal during the Fall '07 term at SUNY Stony Brook.
- Fall '07