lab4 data collection 1

lab4 data collection 1 - gression for Column C...

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Activity 2A: Standard Curve Tube # Absorbance Blank 0 0 Each Group please read your standard tube in one Group's Spec 1 0.03 0.23 to remove the variability between the Specs 2 0.05 0.39 3 0.07 0.51 4 0.08 0.71 5 0.1 0.76 6 0.13 1.28 The tubes for the standard curve were made by mixing known concentrations of L-cysteine with DTNB to produce TNB anion (yellow). Concentration of cysteine (mM) The mM of product thiocholine can be extrapolated from this curve because thiocholine has one sulfhydryl group just as cysteine and reacts similarly with DTNB to produce TNB anion. R-S represents L-cysteine or thiocholine DTNB reacts with sulfhydryl containing compounds, represented here by R-S, to produce the yellow product TNB2- ( Pierce Biotechnology, Inc. 2004). clear yellow 0 0.02 0.04 0.06 0.08 0.1 0.12 0.14 0 0.2 0.4 0.6 0.8 1 1.2 1.4 f(x) = 9.22x - 0.04 Standard Curve Column C Linear Re-
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Unformatted text preview: gression for Column C Concentration of Cysteine (mM) Absorbance Sec# Group# Activity 2B Baseline Experiment Protocol Error: Please note that enzyme is 0.5 ug/ml not 5 ug/ml. Table 4.1 A 1 mg/ml stock is diluted 2000 fold for use in the assay. Reaction Tubes Control Experimental 0.1 M PB7 3.6 ml 3.5 ml Use the control tube to blank the machine 7.5 mM ATCh Use micropipettes to add reagents 5 mM DTNB Total volume 4 ml 4 ml Abs. value expected AChE Activity: Baseline Experiment Reaction conditions Time (sec) A412 nm mM thiocholine Clock Time: 30 temp: 60 90 120 150 180 pH: 210 240 270 300 Group Members: Baseline Experiment Notes: 200 l 200 l 200 l 200 l 0.5 ug/ ml AChE (enzyme) 0 l 100 l Absorbance of Control tube at end of the reaction =...
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This note was uploaded on 10/18/2010 for the course BIO 204 taught by Professor O'neal during the Fall '07 term at SUNY Stony Brook.

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lab4 data collection 1 - gression for Column C...

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