Lab6 Populations Data

Lab6 Populations Data - Section # Should you use one Spec...

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Section # M9 1 1 1:2 2 1:4 3 1:5 4 1:10 5 1:20 6 1:50 Use a dilution of cells that gives an Absorbance between 0.1 and Blank Tube Experimental Tube Blank Tube Should you use one Spec to measure all of the absorbances for the std curve? Why or why not? Group Dilution Stock Cell Culture (saturated) Factor Instructor   4.0 ml   0 ml   2.0 ml   2.0 ml   1.0 ml   3.0 ml   0.8 ml   3.2 ml   0.5 ml   4.5 ml   0.2 ml   3.8 ml   0.1 ml   4.9 ml   How to prepare the Blanks and the Experimental Tubes for a 1:20 dilution The E. coli bacteria are grown in M9+Glc - Why is M9+Glc added to all the blanks including the M9 and LB? How to prepare the Blanks and the Experimental Tubes for a 1:10 dilution The E. coli bacteria are grown in M9+Glc - Why is M9+Glc added to all the blanks including the M9 and LB?
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Experimental Tube If you were given a growth experiment similar to this one, could you deter tubes to use to Blank the Spec?
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Concentration (X) 1 0.5 0.25 0.2 0.1 0.05 0.02 0.2! Calculations for 1:20
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Lab6 Populations Data - Section # Should you use one Spec...

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