M9 Minimal Media Recipe

M9 Minimal Media Recipe - it is able to synthesize all of...

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M9 (glucose minimal salts) broth contains, per liter, 12.8 g Na2HPO4×7H2O 3.1 g KH2PO4 0.5 g NaCl 1.0 g NH4Cl 0.5 g MgSO4×7H2O 4.0 g glucose Most of the nutrients in M9 medium are defined; trace minerals are provided as contaminants in the phosphate salts. E. coli W1485E is able to grow in M9 because
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Unformatted text preview: it is able to synthesize all of the amino acids, nucleotides and coenzymes required for growth and metabolism using glucose as the sole carbon and energy source. M9 is commonly used as a base for preparation of media for nutritional studies on E. coli mutants....
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This note was uploaded on 10/18/2010 for the course BIO 204 taught by Professor O'neal during the Fall '07 term at SUNY Stony Brook.

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