M9 Minimal Media Recipe

M9 Minimal Media Recipe - it is able to synthesize all of...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
M9 (glucose minimal salts) broth contains, per liter, 12.8 g Na2HPO4×7H2O 3.1 g KH2PO4 0.5 g NaCl 1.0 g NH4Cl 0.5 g MgSO4×7H2O 4.0 g glucose Most of the nutrients in M9 medium are defined; trace minerals are provided as contaminants in the phosphate salts. E. coli W1485E is able to grow in M9 because
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: it is able to synthesize all of the amino acids, nucleotides and coenzymes required for growth and metabolism using glucose as the sole carbon and energy source. M9 is commonly used as a base for preparation of media for nutritional studies on E. coli mutants....
View Full Document

Ask a homework question - tutors are online